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Robustness of an analytical procedure is a measure of the analytical method to remain unaffected by small but deliberate variations in method parameters and provides an indication of its reliability during normal usage. The evaluation of robustness is normally considered during the development phase and depends on the type of procedure under study. Experimental design (e.g., fractional factorial design or Plackett Burman design) is common and useful to investigate multiple parameters simultaneously. The result will help to identify critical parameters that will affect the performance of the method. Common method parameters that can affect the analytical procedure should be considered based on the analytical technique and properties of the samples: 1. Sample preparation a. Extraction time b. Sample solvent (pH 0.05 unit, percent organic 2% absolute) c. Membrane lters d. Sample and standard stability 2. High-performance liquid chromatography (HPLC) conditions a. Mobile-phase composition (pH 0.05 unit, percent organic 2% absolute) b. Column used (equivalent columns, lots and/or suppliers, age) c. Temperature d. Flow rate 3. Gas chromatography (GC) conditions a. Column used (lots and/or suppliers, age) b. Temperature c. Flow rate When the results are affected by some critical experimental parameters, a precautionary statement should be included in the analytical procedure to ensure that this parameter is tightly controlled between experiments. For example, if percent ionpairing of mobile phase affects the results signi cantly, the analytical procedure should explicitly be written with a precautionary statement for aqueous component, for example, 40% aqueous 20 mM octanesulfonic acid 2% absolute. Other robustness considerations for ruggedness of the analytical procedure during validation include the following: (a) Sample Extraction Mechanical shaking is preferred over sonication as the latter is affected by a number of factors, for example, water level in bath and position of sample.

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Jan 19, 2016 · Convert Tiff file into PDF file using iTextSharp DLL. iTextSharp.text.pdf.PdfWriter writer = iTextSharp.text.pdf.PdfWriter.GetInstance(document, new System.IO.FileStream(destPdf, System.IO.FileMode.Create)); System.Drawing.Bitmap bmp = new System.Drawing.Bitmap(sourceTif); iTextSharp.text.pdf.PdfContentByte cb = writer ...

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improve the balance). It is true that a balancing ow, calculated via a diffusive algorithm, is incorporated into JOSTLE s re nement algorithm to adjust partitions that become imbalanced, but this is simply a standard tool in JOSTLE s armory, which is frequently never used. Third, and most important, the MLDD scheme is implemented by restricting the matching and then modifying the re nement algorithm, whereas partition inertia is a much more general framework that works by modifying the input graph and, in principle, could use any high-quality partitioning scheme (with the minor requirement that the P xed vertices never migrate from their home subdomain).

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